A novel anticoagulant activity assay of tissue factor pathway inhibitor I (TFPI)

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A novel anticoagulant activity assay of tissue factor pathway inhibitor I (TFPI).

Tissue factor (TF) pathway inhibitor I (TFPI) is the physiological inhibitor of TF-induced blood coagulation. Circulating blood contains full-length TFPI and TFPI truncated at the C-terminal end. Previous studies have shown that full-length TFPI exerts a stronger anticoagulant effect on diluted prothrombin time (DPT) than truncated TFPI, and it has been suggested that full-length TFPI is biolog...

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Recent Progress on Tissue Factor Pathway Inhibitor (TFPI)

外 因 系凝 固 の 開 始 反 応 で あ る組 織 因子 に よ る VII因 子 お よびX因 子 の 活 性 化 を特 異 的 に 阻 害 す る プ ロ テ ア ー ゼ イ ン ヒ ビタ ー の 存 在 は 古 くか ら指 摘 さ れ て きた が,そ の性 質 は長 い 間 明 らか で は な か っ た.そ の後,Brozeら の研 究 に よ り, そ の イ ン ヒ ビタ ー はXa因 子 依 存 性 にVII因 子 の 活 性 化 を阻 害 す る作 用 を もつ とい う一 見,極 め て 奇 妙 な イ ン ヒ ビタ ー で あ る こ とが 明 らか に な っ て きた.1988年 に彼 らに よ り,そ の ア ミノ 酸 配 列 が 決 定 さ れ,そ れ 以 後,い くつ か の グル ー プ に よ り ,そ の組 換 え体 が 調 製 され た ...

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Tissue factor pathway inhibitor anticoagulant activity: risk for venous thrombosis and effect of hormonal state.

Full-length tissue factor pathway inhibitor (TFPI) is assumed to be biologically more important than truncated TFPI because of its stronger anticoagulant effect in the diluted prothrombin time (dPT) assay. We have developed a dPT-based assay for TFPI anticoagulant activity. Here, we report the effect of hormonal state on TFPI anticoagulant activity and whether TFPI anticoagulant activity assess...

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Inhibition of tissue factor pathway inhibitor (TFPI) by ARC19499 improves clotting of hemophiliac blood

Background Hemophilia A and B are disorders resulting from a deficiency in factor VIII (FVIII), and factor IX (FIX), respectively. Tissue Factor (TF) is a key component of the extrinsic pathway and plays a role in the coagulation defect of hemophilic blood. Neutralizing the activity of TFPI represents a promising treatment concept in patients with hereditary or acquired hemophilia. ARC19499 is ...

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Low levels of tissue factor pathway inhibitor (TFPI) increase the risk of venous thrombosis.

There is now strong experimental evidence that tissue factor pathway inhibitor (TFPI) is a critical inhibitor to modulate tissue factor-induced coagulation, but the role of TFPI as a risk factor for thrombosis is yet to be to be determined. This study investigated the role of low TFPI levels for the development of deep-vein thrombosis (DVT). We determined TFPI activity and TFPI-free and total a...

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ژورنال

عنوان ژورنال: Journal of Thrombosis and Haemostasis

سال: 2005

ISSN: 1538-7933,1538-7836

DOI: 10.1111/j.1538-7836.2005.01237.x